研究趋化因子受体4(CXCR4)/趋化因子12(CXCL12)信号途径在食管鳞癌浸润转移中的作用机制，为探讨CXCR4成为食管癌治疗新的靶点提供理论依据。

取对数生长期的食管鳞癌EC9706细胞，添加趋化因子CXCL12，通过侵袭转移实验、黏附实验分别检测食管鳞癌EC9706细胞株的细胞侵袭、移动和黏附能力。采用RT-PCR和Western Blot技术检测表皮生长因子受体(EGFR)mRNA及蛋白的表达水平。

添加不同浓度趋化因子CXCL12(终浓度为5、10 μg/ml)，食管鳞癌EC9706细胞株的细胞侵袭、移动和黏附能力均较空白对照组高，并且存在剂量依存关系，即CXCL12浓度越高，细胞的侵袭、移动、黏附能力越强，差异均有统计学意义(P<0.01)。添加不同浓度趋化因子CXCL12，食管鳞癌细胞的EGFR mRNA和蛋白表达水平均较对照组高，并且存在剂量依存关系，即CXCL12浓度越高，EGFR mRNA和蛋白表达水平越高，差异均有统计学意义(P<0.01)。

CXCR4/CXCL12信号途径与食管鳞癌细胞的侵袭、转移相关，并且存在剂量依存关系，有可能通过调控EGFR的表达参与食管鳞癌的浸润转移。

To explore the mechanism of chemokine receptor 4(CXCR4)/chemotactic factor 12(CXCL12) signaling pathway in invasion and metastasis of esophageal squamous carcinoma so as to provide theoretical basis for CXCR4 as a new target in tumor treatment.

Esophageal squamous carcinoma EC9706 cells in logarithmic phase were treated with CXCL12, and the invasion, migration and adhesion capabilities of EC9706 cells were assessed by the assays of cell adhesion and transwell chamber. RT-PCR and Western Blot were performed to determine the expression of epidermal growth factor receptor(EGFR) mRNA and protein.

The capabilities of invasion, migration and adhesion of EC9706 cells increased with the concentrations of CXCL12(5, 10 μg/ml) in a dose-dependent manner(P<0.01), and were significantly higher than those of blank controls(P<0.01). The expression of EGFR mRNA and protein in EC9706 cells increased with the concentrations of CXCL12 in a dose-dependent manner(P<0.01), and was significantly higher than that of controls(P<0.01).

CXCR4/CXCL12 signaling pathway is related to tumor invasion and metastasis, which exhibits a dose-dependent relationship. CXCR4/CXCL12 signaling pathway may participate in the invasion and metastasis of esophageal squamous cell carcinoma via regulating the expression of EGFR.