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中华胸部外科电子杂志 ›› 2020, Vol. 07 ›› Issue (03) : 152 -158. doi: 10.3877/cma.j.issn.2095-8773.2020.03.05

所属专题: 文献

论著

滤泡辅助性T细胞在重症肌无力伴胸腺瘤患者胸腺中的表达
宋阳1, 叶晓玲1, 陈佶1, 朱勇俊1, 徐朋亮2, 伍宁1, 陈刚1, 苗锋1, 巫伟伟1, 陈志明1,()   
  1. 1. 200040 上海,复旦大学附属华山医院胸外科
    2. 313000 湖州,湖州市第一人民医院胸外科
  • 收稿日期:2020-05-20 修回日期:2020-06-22 接受日期:2020-07-02 出版日期:2020-08-28
  • 通信作者: 陈志明
  • 基金资助:
    复旦大学附属华山医院北院院内启动基金(HSBY2017005)

Expression of T follicular helper cells in myasthenia gravis patients with thymoma

Yang Song1, Xiaoling Ye1, Ji Chen1, Yongjun Zhu1, Pengliang Xu2, Ning Wu1, Gang Chen1, Feng Miao1, Weiwei Wu1, Zhiming Chen1,()   

  1. 1. Department of Thoracic Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China
    2. Department of Thoracic Surgery, Huzhou First People’s Hospital, Huzhou 313000, China
  • Received:2020-05-20 Revised:2020-06-22 Accepted:2020-07-02 Published:2020-08-28
  • Corresponding author: Zhiming Chen
  • About author:
    Corresponding author: Chen Zhiming, Email:
引用本文:

宋阳, 叶晓玲, 陈佶, 朱勇俊, 徐朋亮, 伍宁, 陈刚, 苗锋, 巫伟伟, 陈志明. 滤泡辅助性T细胞在重症肌无力伴胸腺瘤患者胸腺中的表达[J]. 中华胸部外科电子杂志, 2020, 07(03): 152-158.

Yang Song, Xiaoling Ye, Ji Chen, Yongjun Zhu, Pengliang Xu, Ning Wu, Gang Chen, Feng Miao, Weiwei Wu, Zhiming Chen. Expression of T follicular helper cells in myasthenia gravis patients with thymoma[J]. Chinese Journal of Thoracic Surgery(Electronic Edition), 2020, 07(03): 152-158.

目的

探讨滤泡辅助性T细胞(Tfh)在重症肌无力(MG)伴胸腺瘤患者胸腺组织中的表达变化。

方法

采用免疫荧光染色技术、免疫组织化学法和蛋白质印迹法,检测30例MG伴胸腺瘤患者(MG组)、20例无MG的胸腺瘤患者(NMG组)的瘤旁组织及10例心脏手术患者(对照组)萎缩胸腺组织中的Tfh细胞比例及其蛋白分子CXCR-5、ICOS、PD-1和Bcl-6的表达水平。

结果

与NMG组和对照组相比,MG组胸腺组织中Tfh细胞比例及其CXCR-5、ICOS、PD-1和Bcl-6的表达水平显著增高,差异有统计学意义(P<0.05);NMG组胸腺组织中Tfh细胞比例及其CXCR-5、ICOS表达水平显著高于对照组,差异有统计学意义(P<0.05);NMG组与对照组胸腺组织中Tfh细胞PD-1和Bcl-6的表达水平差异无统计学意义(P>0.05)。

结论

MG伴胸腺瘤患者瘤旁胸腺组织中的Tfh可能通过提高自身免疫活性参与MG的发生和进展,抑制Tfh信号通路可能为MG的治疗提供新途径。

Objective

To investigate role of thymic T follicular helper (Tfh) cells in the immunopathogenesis of myasthenia gravis (MG) patients with thymoma.

Methods

The percentage of Tfh cells were detected in 30 MG patients with thymoma (MG group), 20 non-MG patients with thymoma (NMG group) and 10 individuals with atrophic thymus (control group). The marker protein CXCR-5, ICOS, PD-1 and Bcl-6 were analyzed by immunofluorescence, immunohistochemical staining, and Western blotting.

Results

Compared with the NMG group and the control group, the Tfh cells and the expression of CXCR-5, ICOS, PD-1 and Bcl-6 protein were increased, the differences were statistically significant (P<0.05). The percentage of Tfh cells and the expression of CXCR-5, ICOS in the NMG group were significantly higher than those in the control group (P<0.05); there was no significant difference in the expression of PD-1 and Bcl-6 between the NMG group and the control group (P>0.05).

Conclusion

Tfh cells in the peritumoral thymus of the MG patients with thymoma may involve in the generation and development of MG via enhancing immune activity. Inhibiting the immune function of Tfh cells may provide a new strategy approaching for the treatment of MG.

表1 三组患者的临床资料
图1 CD4+CXCR5+Tfh细胞在胸腺瘤伴MG患者中高表达
图2 Tfh细胞膜蛋白CXCR5和PD-1在MG伴胸腺瘤中高表达
图3 胸腺组织中Tfh细胞蛋白分子Bcl-6和COS的表达差异
图4 Bcl-6和PD-1蛋白质印迹法检测结果
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