Expression of TPX2 in esophageal gastric junction adenocarcinoma and its clinical significance

doi:10.3877/cma.j.issn.2095-8773.2015.04.005

Abstract

Abstract:

Objective

To investigate the expression of TPX2 in esophageal gastric junction adenocarcinoma and its clinic significance.

Methods

Immunohistochemistry was used to detect the expression of TPX2 protein in 80 cases of esophageal gastric junction adenocarcinoma, 41 cases of adjacent atypical hyperplasia tissues and 48 cases of normal gastric mucosa tissues. RT-PCR was adopted to determine the expression of TPX2 mRNA in 40 cases of esophageal gastric junction adenocarcinoma, 36 cases of adjacent atypical hyperplasia tissues and 28 cases of normal gastric mucosa tissues.

Results

The positive rates of TPX2 protein expression in esophageal gastric junction adenocarcinoma, adjacent atypical hyperplasia tissues and normal gastric mucosa tissues were 71.2% (57/80), 53.7% (22/41) and 31.2% (15/48), respectively, and there were significant differences among them(χ²=10.315, P=0.018). The positive rates of TPX2 mRNA expression were 77.5%(31/40), 69.4%(25/36) and 7.1%(2/28), respectively, and there were significant differences among them(χ²=7.468, P=0.025). The positive rates of expression of TPX2 protein and mRNA in esophageal gastric junction adenocarcinoma increased with the infiltration depth, lymph node metastasis and tumor staging(P<0.05), while were not related to sex, age and tissue differentiation (P>0.05).

Conclusions

The abnormal expression of TPX2 may be involved in early carcinogenesis, infiltration and metastasis of esophageal gastric junction adenocarcinoma, and the detection of TPX2 expression may play an important role in the early diagnosis and prognosis prediction in patients with esophageal gastric junction adenocarcinoma.

Key words: TPX2, Esophageal gastric junction adenocarcinoma, RT-PCR, Immunohistochemistry

Liang Shen, Yiming Xu, Shengguang Ding, Chongjun Zhong. Expression of TPX2 in esophageal gastric junction adenocarcinoma and its clinical significance[J]. Chinese Journal of Thoracic Surgery(Electronic Edition), 2015, 02(04): 232-236.

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Figures/Tables 4

References 12

[6]	Lin DM, Ma Y, Xiao T，et al.TPX2 expression and its significance in esophageal squamous cell carcinoma[J]．Zhonghua Bing Li Xue Za Zhi，2015，35(9)：540.
[7]	Shigeishi H, Ohta K, Hiraoka M，et al.Expression of TPX2 in salivary gland carcinomas[J]．Oncol Rep，2009，21: 341-344.
[8]	Warner SL, Stephens BJ, Nwokenkwo S，et al.Validation of TPX2 as a potential therapeutic target in pancreatic cancer cells[J]．Clin Cancer Res，2015，15: 6519-6528.
[9]	Vos JW, Pieuchot L, Evrard JL, et al. The Plant TPX2 protein regulates prospindle assembly before nuclear envelope breakdown[J]．Plant Cell, 2008, 20(10): 2783.
[10]	常海平，王敬芝，田原，等．宫颈鳞癌组织中TPX2的表达及其临床意义[J]．中国实用妇科与产科杂志，2012，28(9)：685-687.
[11]	Gritsko TM, Coppola D, Paciga JE，et al.Activation and over expression of centrosome kinase BT AK Aurora A in human ovarian cancer[J]．Clin Cancer Res，2003，9(4)：1420-1429.
[1]	Zhi H, Zhang J, H u G，et al.The deregulation of arachidonic acid metabolism related genes in human esophageal squamous cell carcinoma[J]．Int J Cancer, 2015，106(3)：327-331.
[2]	Ma Y, Lin D, Sun W, et al.Expression of targeting protein for xklp2 associated with both malignant transformation of respiratory epithelium and progression of squamous cell lung cancer [J]．J Clin Cancer Res，2014，12(4)：1121-1127.
[3]	林冬梅，马莹，肖汀，等．TPX2在肺鳞状细胞癌及其癌前病变中的表达和意义[J]. 中华病理学杂志，2006，35(9)：504.
[4]	刘红春，李莹莹，刘玉含，等．食管鳞癌组织中TPX2mRNA的表达及与临床病理生物学特征的关系[J]．临床与实验病理学杂志，2010，26(2)：708-710.
[5]	Tong T, Zhong Y, Kong J，et al.Over expression of Aurora A contributes to malignant development of human esophageal squamous cell carcinoma[J] . Clin Cancer Res，2014，10(21)：304-306.
[12]	Krams M, Heidebrecht HJ, Hero B，et al.Repp86 expression and outcome in patients with esophageal squamous cell carcinoma[J]．J Clin Onco，2003，21(9): 1810.

不同组织类型	TPX2蛋白				TPX2 mRNA
不同组织类型	总例数	阳性率	χ²值	P值	总例数	阳性率	χ²值	P值
正常胃黏膜组织	48	31.2%(15/48))	?	?	28	7.1%(2/28)	?	?
癌旁不典型增生组织	41	53.7%(22/41)	10.315	0.018	36	69.4%(25/36)	7.468	0.025
食管胃交界部腺癌	80	71.2%(57/80)	?	?	40	77.5%(31/40)	?	?

不同组织类型	TPX2蛋白				TPX2 mRNA
不同组织类型	总例数	阳性率	χ²值	P值	总例数	阳性率	χ²值	P值
正常胃黏膜组织	48	31.2%(15/48))	?	?	28	7.1%(2/28)	?	?
癌旁不典型增生组织	41	53.7%(22/41)	10.315	0.018	36	69.4%(25/36)	7.468	0.025
食管胃交界部腺癌	80	71.2%(57/80)	?	?	40	77.5%(31/40)	?	?

临床特征		TPX2蛋白的表达				TPX2 mRNA的表达
临床特征		总例数	阳性率	χ²值	P值	总例数	阳性率	χ²值	P值
性别		?	?	?	?	?	?	?	?
?	男性	59	69.5%(41/59)	0.010	0.919	33	78.8%(26/33)	0.047	0.829
?	女性	21	76.2%(16/21)	0.010	0.919	7	71.4%(5/7)	0.047	0.829
年龄(岁)		?	?	?	?	?	?	?	?
?	≥60	43	69.8%(30/43)	0.190	0.663	26	80.8%(21/26)	0.612	0.434
?	<60	37	73.0%(27/37)	0.190	0.663	14	71.4%(10/14)	0.612	0.434
肿瘤分化程度		?	?	?	?	?	?	?	?
?	高分化	27	74.1%(20/27)	0.012	0.826	11	72.7%(8/11)	0.298	0.585
?	中分化	32	68.7%(22/32)			16	87.5%(14/16)
?	低分化	21	71.4%(15/21)			13	69.2%(9/13)
肿瘤浸润深度		?	?	?	?	?	?	?	?
?	黏膜层	14	42.9%(6/14)	5.423	0.025	6	33.3%(2/6)	2.183	0.038
?	肌层	29	69.0%(20/29)			15	73.3%(11/15)
?	浆膜层	37	83.8%(31/37)			19	94.7%(18/19)
淋巴结转移		?	?	?	?	?	?	?	?
?	有	69	76.8%(53/69)	8.683	0.000	34	85.3%(29/34)	9.125	0.026
?	无	11	36.4%(4/11)	8.683	0.000	6	33.3%(2/6)	9.125	0.026
肿瘤TMN分期		?	?	?	?	?	?	?	?
?	Ⅰ期	9	22.2%(2/9)	23.234	0.045	2	50.0%)1/2)	15.369	0.004
?	Ⅱ期	33	63.6%(21/33)			12	66.7%(8/12)
?	Ⅲ期	38	89.5%(34/38)			26	84.6%(22/26)

临床特征		TPX2蛋白的表达				TPX2 mRNA的表达
临床特征		总例数	阳性率	χ²值	P值	总例数	阳性率	χ²值	P值
性别		?	?	?	?	?	?	?	?
?	男性	59	69.5%(41/59)	0.010	0.919	33	78.8%(26/33)	0.047	0.829
?	女性	21	76.2%(16/21)	0.010	0.919	7	71.4%(5/7)	0.047	0.829
年龄(岁)		?	?	?	?	?	?	?	?
?	≥60	43	69.8%(30/43)	0.190	0.663	26	80.8%(21/26)	0.612	0.434
?	<60	37	73.0%(27/37)	0.190	0.663	14	71.4%(10/14)	0.612	0.434
肿瘤分化程度		?	?	?	?	?	?	?	?
?	高分化	27	74.1%(20/27)	0.012	0.826	11	72.7%(8/11)	0.298	0.585
?	中分化	32	68.7%(22/32)			16	87.5%(14/16)
?	低分化	21	71.4%(15/21)			13	69.2%(9/13)
肿瘤浸润深度		?	?	?	?	?	?	?	?
?	黏膜层	14	42.9%(6/14)	5.423	0.025	6	33.3%(2/6)	2.183	0.038
?	肌层	29	69.0%(20/29)			15	73.3%(11/15)
?	浆膜层	37	83.8%(31/37)			19	94.7%(18/19)
淋巴结转移		?	?	?	?	?	?	?	?
?	有	69	76.8%(53/69)	8.683	0.000	34	85.3%(29/34)	9.125	0.026
?	无	11	36.4%(4/11)	8.683	0.000	6	33.3%(2/6)	9.125	0.026
肿瘤TMN分期		?	?	?	?	?	?	?	?
?	Ⅰ期	9	22.2%(2/9)	23.234	0.045	2	50.0%)1/2)	15.369	0.004
?	Ⅱ期	33	63.6%(21/33)			12	66.7%(8/12)
?	Ⅲ期	38	89.5%(34/38)			26	84.6%(22/26)

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