The cases of double sleeve uniportal thoracoscopic lobectomy in Shanghai Pulmonary Hospital were retrospectively analyzed and the key points of operational technique were summarized, to further discuss and explore the operation effects and application values.

A total of 47 patients who underwent double sleeve uniportal thoracoscopic lobectomy in Shanghai Pulmonary Hospital from June 2015 to January 2021 were consecutively enrolled. Patients were followed up remotely by telephone every 6 months post-operation. The operation duration, intraoperative bleeding, drainage volume on the first day after operation, postoperative hospital stay, complications and 1- and 2-year survival rates were recorded and analyzed.

A total of 41 males and 6 females were enrolled, with an age of (60.5±8.0) years. 41 (87.2%) cases received left upper lobe double sleeves lobectomy, 3 (6.4%) cases received right upper lobe double sleeves lobectomy and 3 (6.4%) cases received right and middle double sleeves bilobectomy. The main pathological type was squamous cell carcinoma (30, 63.8%) . There were 9 (19.1%) , 17 (36.2%) and 21 (44.7%) of patients who had TNM stages of stage I, II and III, respectively. The mean operation duration was (2.7 ± 0.8) h, the mean intraoperative blood loss was (168.1±160.0) mL, and the mean postoperative hospital stay was (5.9±2.9) days. There was no case conversing to thoracotomy. The median follow-up time was 27 months. The 1-year overall survival rate was 87.9% and the 2-year overall survival rate was 68.7%.

Double sleeve uniportal thoracoscopic lobectomy can be used to completely remove tumors and preserve lung function to the greatest extent with reduced surgical traumas. This operation is technique-demanding, which is feasible and safe when carried out in thoracic centers with endoscopy proficiency.

To access the efficacy and safety of minimally invasive surgery of modified Nuss technique without being turned over for the treatment of adult recurrent pectus excavatum.

From July 2008 to September 2021, 48 adult recurrent pectus excavatum cases were treated with minimally invasive surgery of modified Nuss technique without being turned over in the Department of Thoracic Surgery of Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine and in the Department of Cardiothoracic Surgery of Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, among which 22 cases were performed previously by Ravitch procedure, 23 cases were Nuss procedure, 2 cases were Ravitch combined with Nuss procedure, 1 case was uncertain respectively.

All 48 cases were performed by minimally invasive surgery of modified Nuss technique without being turned over successfully. The average postoperative hospital stays were 5.58 days. The Hallex index was improved. The complications were mild. The patients were all satisfied with their chest appearance after the operation.

Minimally invasive surgery of modified Nuss technique without being turned over is effective and safe, which will be a surgical choice for the treatment of adult recurrent pectus excavatum in the future.

To display the anatomy of swine lung lobes, especially the characteristics of bronchus, pulmonary arteries and pulmonary veins of each lung lobe, in order to provide reference for animal experiments of lobectomy.

The heart and lung organs of an adult pig were extracted to observe the structure of the lung lobes. Further, the lung of the pig was dissected at the level of lung lobes to observe the anatomical characteristics of the bronchus, pulmonary arteries and pulmonary veins.

Swine lungs are divided into left and right lungs. The left lung has 3 lobes, namely apical lobe, cardial lobe and diaphragmatic lobe, in which the left apical lobe and left cardial lobe are fused. The right lung has 4 lobes, namely apical lobe, cardial lobe, diaphragmatic lobe and accessory lobe. Each lobe has independent bronchus, pulmonary artery and pulmonary vein. The left apical lobe and left cardial lobe bronchus are co-stem, which is similar to the human left upper lobe bronchus. Two pulmonary arteries branch into the left apical lobe, and only one pulmonary artery branch into the left diaphragmatic lobe and each lobe of the right lung. There are two pulmonary veins in the right accessory lobe. One of the accessory pulmonary veins joins at the root of the pulmonary veins in the diaphragmatic lobe and enters the pericardium, while any of other pulmonary lobes has only one pulmonary vein.

The anatomical characteristics of bronchus, pulmonary arteries and pulmonary veins of swine lung are similar to those of human lung, and can be used as an animal model for lobectomy. Familiarity with the anatomy of swine lung is helpful to carry out relevant experiments and studies.

For early-stage thymic epithelial tumors (TETs) , advocation for partial thymectomy or even thymomectomy has always existed. The concept of hemi-thymectomy is based on the anatomical structure of the thymus; and it is devised to be applied to early-stage TETs and benign thymic lesions located entirely on one lobe of the thymus. This article summarized our preliminary experience with this novel surgical extent including both peri-operative outcomes and short-term prognosis.

Over a 12-month period, a total of 31 patients underwent minimally invasive hemi-thymectomy. The clinical characteristics, co-morbidities, peri-operative results, final pathology and short-term prognosis of these patients were summarized.

A total of 11 males and 20 females with a mean age of 52.7 (35–77) years underwent minimally invasive hemi-thymectomy for their anterior mediastinal lesions. Twenty-six of them had various degrees of co-morbidities: 12 with overweight, 9 with hypertension, 3 with diabetes mellitus, 1 with leukopenia and 1 with endometrial stromal sarcoma. All 31 hemi-thymectomies via the anterolateral minimally invasive approach were successful without intra-operative adverse events. The mean operation time was 60.83 (31–91) min. The mean intra-operative blood loss was 49.5 (20–200) mL. There were no deaths in peri-operative period. The mean post-operative hospital stay was 1.3 (1–2) days; the mean drainage volume on the first post-operative day was 77.8 (0–350) mL. The mean chest drainage tube indwelling time was 0.7 (0–2) days. Drainage tubes of 10 patients were removed within 24 hours after the surgery. The pathology results showed 21 thymomas, 7 teratomas and 3 hemangiomas. All 21 thymomas were UICC pT1aN0M0. No recurrence was observed during the follow-up (6–12 months) .

Hemithymectomy, a new range of thymectomy based on thymic anatomy, is used to treat early-stage, non-myasthenia gravis-derived tumors of thymic epithelial origin and benign lesions located in one thymus. Preliminary exploration suggests that this is a safe and effective resection method with good perioperative and short-term oncological outcomes. In theory, hemithymectomy has inherent advantages over traditional total thymectomy, and can provide a faster and safer surgical experience for a selected group of patients.

To investigate the effect of protein regulator of cytokinesis 1 (PRC1) on lymph node metastasis, migration, proliferation and prognosis of lung adenocarcinoma.

The non-small cell lung cancer expression matrix was obtained from Gene Expression Omnibus (GEO), including GSE7670, GSE32863 and GSE68465. Firstly, differential analysis was performed on the three datasets to obtain differentially expressed genes, which were then subjected to enrichment analysis. Secondly, the clinical and expression data of differentially expressed genes were obtained from The Cancer Genome Atlas (TCGA) database. The data was used for weighted gene co-expression network analysis (WGCNA) to obtain the module most relevant to clinical characteristics including tumor stage and lymph node metastasis. Thirdly, the hub gene was obtained through analysis of the module genes using the Search Tool for the Retrieval of Interaction Genes/Proteins (STRING) and binary logistic regression analysis. Subsequently, the expression level of PRC1 in both mRNA and protein levels between lung adenocarcinoma and normal tissues was validated. After knocking down the expression of PRC1 in human lung adenocarcinoma cell line H1299, in vitro functional experiments were conducted to validate the impact of PRC1 on the proliferation, migration, and invasion abilities of lung adenocarcinoma cells. T-test was used to compare the difference of PRC1 expression between normal and tumor tissues, and binary logistic regression analysis was performed to identify independent factors affecting tumor prognosis. Additionally, the downstream mechanism of PRC1 in regulating the metastasis of lung adenocarcinoma cells was investigated.

The expression level of PRC1 was higher in lung adenocarcinoma tissues compared to normal lung tissues, at both mRNA and protein levels, with statistical significance. The cells in the Si-PRC1 group were significantly lower than that in the control group in migration experiments (P<0.001), invasion experiments (P<0.001), and scratch experiments (P<0.001). The proliferation ability of Si-PRC1 group cells was significantly lower than that of the control group at 72 and 96 hours. Patients with high expression of PRC1 in lung adenocarcinoma had shorter overall survival (P=0.0014). The qPCR results showed that E-cadherin expression was significantly upregulated (P<0.001) in PRC1 knockdown cells, while N-cadherin and Vimentin expression were significantly downregulated (P<0.01). Western blot results showed that the activated phosphorylated MAPK signal was weakened in PRC1 knockout tumor cells.

PRC1 is associated with poor prognosis of LUAD patients and affects lymph node metastasis and tumor cells proliferation, migration and invasion. PRC1 is a potential therapeutic target for lung adenocarcinoma.

To retrospectively analyze the patients who underwent uniportal thoracoscopic right upper lobectomy using double-stapling vs traditional technique at Anning First People's Hospital Affiliated to Kunming University of Science and Technology. Compare the differences between the double-stapling technique and traditional technique in the application of uniportal thoracoscopic right upper lobe resection, summarize the key points of the double-stapling surgical technique, and explore the surgical results and promotional value.

Forty-nine patients from July 2021 to May 2023 were consecutively enrolled at Anning First People's Hospital. A total of 22 patients underwent uniportal thoracoscopic two-stapler resection of the right upper lobe. A total of 27 patients underwent uniportal thoracoscopic right upper lobectomy using traditional methods. The outcome measures were operative time, intraoperative bleeding, drainage tube duration and postoperative hospital stay.

For the double-stapling technique group, 22 patients (16 males, 6 females) were enrolled. The median age was (58.00±9.38) years old. There were no cases converted to thoracotomy. The mean operative duration was (84.86±15.81) min, the mean intraoperative blood loss was (73.86±21.98) mL, the mean drainage tube duration was 2.00 (1.00, 2.00) days, and the mean postoperative hospital stay was 5.00 (4.75, 6.00) days. For the traditional technique group, 27 patients (13 males, 14 females) were enrolled. The median age was (58.46±8.63) years old. The mean operative duration was (93.55±13.43) min, the mean intraoperative blood loss was (85.34±20.22) mL, the mean drainage tube duration was 2.00 (1.00, 3.00) days, and the mean postoperative hospital stay was 5.00 (4.50, 6.00) days. The shorter surgical operative time for the double-stapling technique was statistically significant (P<0.05) .

Compared to traditional techniques, uniportal thoracoscopic double-stapling technique for right upper lobectomy has a shorter operative time while also achieving a curative effect. The operative process is simple, easy to perform, safe, reliable, and benefits patients while reducing their economic burden.