Effects of hydrogen sulfide on function change of isolated pulmonary artery in rat lung transplantation model

doi:10.3877/cma.j.issn.2095-8773.2017.01.05

Abstract

Abstract:

Objective

To investigate the effects of hydrogen sulfide on function change of isolated pulmonary artery in rat lung transplantation model.

Methods

Male SD rats were randomly divided into control group(sham operation group), lung transplantation group and lung transplantation+ hydrogen sulfide group, with 10 rats in each group. In hydrogen sulfide+ lung transplantation group, orthotopic left lung allograft transplantation was performed, and hydrogen sulfide 14 μmol/kg was injected intraperitoneally at the beginning of reperfusion. Rats were sacrificed 2 h after reperfusion, and left lung tissues were harvested to determine the wet dry ratio (W/D), content of malondialdehyde (MDA) and activity of myeloperoxidase(MPO) and inducible nitric oxide synthase (iNOS). Isolated pulmonary artery rings were prepared, and the vasodilation and contraction function of pulmonary artery rings were compared.

Results

The W/D, MDA content and activity of MPO and iNOS in lung transplantation group were significantly higher than those in control group(P<0.05). The W/D, MDA content and activity of MPO and iNOS in lung transplantation+ hydrogen sulfide group were significantly lower than those in lung transplantation group (P<0.05). Compared with control group, the endothelium-dependent vasodilation function of isolated pulmonary artery significantly decreased in lung transplantation group (P<0.05), but after hydrogen sulfide treatment, the vasodilation function restored to the level in control group (P>0.05). The contraction function of isolated pulmonary artery in lung transplantation group was significantly lower than that in control group (P<0.05), but there was no significant change in contraction function of isolated pulmonary artery in lung transplantation+ hydrogen sulfide group (P>0.05).

Conclusions

Hydrogen sulfide can alleviate the oxidative stress and inflammatory reaction, inhibit the activity of iNOS, reduce the ischemia-reperfusion injury after lung transplantation, and improve the vasodilation function of isolated pulmonary artery after transplantation.

Key words: Hydrogen sulfide, Lung transplantation, Ischemia-reperfusion injury, isolated pulmonary artery, nitric oxide synthase

Hongwei Zhu, Jingxiang Wu, Meiying Xu. Effects of hydrogen sulfide on function change of isolated pulmonary artery in rat lung transplantation model[J]. Chinese Journal of Thoracic Surgery(Electronic Edition), 2017, 04(01): 22-26.

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Figures/Tables 2

References 15

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组别		W/D	MDA(ng/ml)	MPO(ng/ml)	iNOS(ng/ml)	eNOS(ng/ml)
对照组^①		4.2±0.5	13.3±0.5	1.0±0.2	0.3±0.1	0.7±0.2
肺移植组^②		5.2±0.3	21.2±4.2	1.2±0.1	0.8±0.1	0.3±0.1
硫化氢-肺移植组^③		4.6±0.3	16.3±2.1	1.1±0.1	0.5±0.1	0.5±0.1
①与②比较		?	?	?	?	?
?	t值	－5.42	－5.91	－2.83	－11.18	5.66
?	P值	P<0.05	P<0.05	P<0.05	P<0.05	P<0.05
②与③比较		?	?	?	?	?
?	t值	4.47	3.30	2.24	6.71	－4.47
?	P值	P<0.05	P<0.05	P<0.05	P<0.05	P<0.05

组别		W/D	MDA(ng/ml)	MPO(ng/ml)	iNOS(ng/ml)	eNOS(ng/ml)
对照组^①		4.2±0.5	13.3±0.5	1.0±0.2	0.3±0.1	0.7±0.2
肺移植组^②		5.2±0.3	21.2±4.2	1.2±0.1	0.8±0.1	0.3±0.1
硫化氢-肺移植组^③		4.6±0.3	16.3±2.1	1.1±0.1	0.5±0.1	0.5±0.1
①与②比较		?	?	?	?	?
?	t值	－5.42	－5.91	－2.83	－11.18	5.66
?	P值	P<0.05	P<0.05	P<0.05	P<0.05	P<0.05
②与③比较		?	?	?	?	?
?	t值	4.47	3.30	2.24	6.71	－4.47
?	P值	P<0.05	P<0.05	P<0.05	P<0.05	P<0.05

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