Expression and clinical significance of miR-125b in esophageal squamous cell carcinoma

doi:10.3877/cma.j.issn.2095-8773.2019.02.06

Abstract

Abstract:

Objective

To detect the expression of miR-125b in esophageal squamous cell carcinoma ( ESCC) tissues and differentiated ESCC cells , and to analyze its correlation with clinicopathological parameters and prognosis of ESCC patients .

Methods

ESCC cell lines including KYSE410 ,TE-13 ,EC9706 ,KYSE510 and human normal esophageal cell lines , Het-1A were cultured in vitro .The clinical data of 89 cases of esophageal squamous cell carcinoma and adjacent tissues were collected .The expression of miR-125b in the above tissues and cells was detected by qRT-PCR . The clinicopathological parameters and follow-up data of ESCC patients were collected , and the correlation between the expression of miR-125b in tissue samples and clinicopathological parameters and prognosis was analyzed .

Results

The expression of miR-125b in ESCC cells was significantly lower than that in human normal esophageal cells ( P＜0 .05) ,and the expression of miR-125b in low-differentiated ESCC cells (KYSE410 ,TE-13) was significantly lower than that in highly differentiated ESCC cells (EC 9706 ,KYSE510) ( P＜0 .05) . The expression of miR-125b in ESCC tissues was also significantly lower than that in adjacent tissues ( P＜0 .05) ,and the low expression of miR-125b was closely related to pTNM ,lymph node metastasis and the degree of differentiation of tumors ( P＜0 .05) .Survival analysis showed that the prognosis of patients with low expression of miR-125b was poor ( P＜0 .05) . Cox regression analysis showed that low expression of miR-125b was an independent prognostic factor in patients with ESCC ( P＜0 .05) .

Conclusions

The abnormal low expression of miR-125b is closely related to the occurrence and progression of ESCC , and is an independent risk factor affecting the prognosis of patients .

Key words: Esophageal neoplasms, Squamous cell carcinoma, miR-125b

Yusong Fang, Junling Liu, Zhiping Zhang, Zongming Wang, Liguo Yin, Peng Xu. Expression and clinical significance of miR-125b in esophageal squamous cell carcinoma[J]. Chinese Journal of Thoracic Surgery(Electronic Edition), 2019, 06(02): 106-110.

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URL: https://zhxbwkdzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-8773.2019.02.06

https://zhxbwkdzzz.cma-cmc.com.cn/EN/Y2019/V06/I02/106

Figures/Tables 4

References 17

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参数	低表达组（Ｎ＝45）	高表达组（Ｎ＝44）	χ²值	Ｐ值
性别			0.279	0.597
男性	32（52.46）	29（47.54）
女性	13（46.43）	15（53.57）
年龄/岁			1.465	0.226
≥60性	40（53.33）	35（46.67）
＜60性	5（35.71）	9（64.29）
分化程度			5.298	0.021
中高分化	22（40.74）	32（59.26）
低分化	23（65.71）	12（34.29）
肿瘤长度			2.589	0.108
≥5cm	24（60.00）	16（40.00）
＜5cm	21（42.86）	28（57.14）
pTNM分期			5.951	0.015
Ⅰ期＋ⅡA期	17（37.78）	28（62.22）
ⅡB期＋Ⅲ期	28（63.64）	16（36.36）
淋巴结转移			8.461	0.004
有	26（68.42）	12（31.58）
无	19（37.25）	32（62.75）

参数	低表达组（Ｎ＝45）	高表达组（Ｎ＝44）	χ²值	Ｐ值
性别			0.279	0.597
男性	32（52.46）	29（47.54）
女性	13（46.43）	15（53.57）
年龄/岁			1.465	0.226
≥60性	40（53.33）	35（46.67）
＜60性	5（35.71）	9（64.29）
分化程度			5.298	0.021
中高分化	22（40.74）	32（59.26）
低分化	23（65.71）	12（34.29）
肿瘤长度			2.589	0.108
≥5cm	24（60.00）	16（40.00）
＜5cm	21（42.86）	28（57.14）
pTNM分期			5.951	0.015
Ⅰ期＋ⅡA期	17（37.78）	28（62.22）
ⅡB期＋Ⅲ期	28（63.64）	16（36.36）
淋巴结转移			8.461	0.004
有	26（68.42）	12（31.58）
无	19（37.25）	32（62.75）

因素	ＨＲ	95％ＣＩ	Ｐ值
性别	1.449	0.901～2.421	0.119
年龄	1.126	0.757～1.897	0.178
分化程度	0.724	0.495～1.214	0.226
肿瘤长度	1.354	0.799～2.250	0.137
pTNM分期	2.268	1.517～4.014	0.031
淋巴结转移	2.781	1.716～4.436	0.014
miR-125b表达	3.147	2.358～5.578	0.009

因素	ＨＲ	95％ＣＩ	Ｐ值
性别	1.449	0.901～2.421	0.119
年龄	1.126	0.757～1.897	0.178
分化程度	0.724	0.495～1.214	0.226
肿瘤长度	1.354	0.799～2.250	0.137
pTNM分期	2.268	1.517～4.014	0.031
淋巴结转移	2.781	1.716～4.436	0.014
miR-125b表达	3.147	2.358～5.578	0.009

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